Found in Yasni Exposé of Gargi Sen

D. N. Roy, S. Mandal, G. Sen, S. Mukhopadhyay, T. Biswas. British Journal of Pharmacology, Vol. 9999, No. 999A. (2010). Background and purpose: Andrographis paniculata (AP) has been found to display hepatoprotective effect, although the mechanism of action of the active compounds of AP in this context still remains unclear. Here, we evaluated the hepatoprotective efficacy of 14-deoxyandrographolide (14-DAG), a bioactive compound of AP, paticularly its role in desensitization of hepatocytes to TNF03B1-induced signalling of apoptosis.Experimental approach: TNF03B1-mediated ligand receptor interaction in hepatocytes in presence of 14-DAG was studied in vitro in primary hepatocyte cultures, with the help of co-immunoprecipitation, confocal microscopy and FACS analysis. Events associated with 14-DAG-induced TNFRSF1A release from hepatocytes were determined using immunoblotting, biochemical assay and fluorimetric studies. Pulse-chase experiments with radiolabelled TNF03B1 and detection of apoptotic nuclei by TUNEL labelling were performed under in vivo conditions.Key results: 14-DAG down-regulated the formation of death-inducing signalling complex (DISC), resulting in desensitization of hepatocytes to TNF03B1-induced apoptosis. Pretreatment of hepatocytes with 14-DAG accentuated microsomal Ca-ATPase activity through induction of NO/cGMP pathway. This resulted in enhanced calcium influx into microsomal lumen with the formation of TNFRSF1A-ARTS-1-NUCB2 complex in cellular vesicles. It was followed by the release of full length 55kDa TNFRSF1A and a reduction in the number of cell surface TNFRSF1A, which eventually caused diminution of TNF03B1 signal in hepatocytes.Conclusion and Implication: Taken together, the results demonstrate for the first time that 14-DAG desensitizes hepatocytes to TNF03B1-mediated apoptosis through the release of TNFRSF1A. This can be used as a strategy against cytokine-mediated hepatocyte apoptosis in liver dysfunctions.

ResearchGATE is a scientific network that connects researchers. Find research partners, collaborate with scientists and explore journal articles.

ResearchGATE is a scientific network that connects researchers. Find research partners, collaborate with scientists and explore journal articles.

ResearchGATE is a scientific network that connects researchers. Find research partners, collaborate with scientists and explore journal articles.

CABI Abstract | Combination of ascorbate and α-tocopherol as a preventive therapy against structural and functional defects of erythrocytes in visceral leishmaniasis. | The redox unbalance in erythrocytes has been found to contribute significantly in the development of anaemia in visceral leishmaniasis (VL). This study revealed the enhanced production of reactive | Gargi Sen, Rupa Mukhopadhaya, Jharna Ghosal, Tuli Biswas | Free Radical Research