Number,protein in Yasni Exposé of Akira Murasugi

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Akira Murasugi, 75, Scientific Researcher, Odawara

Country: Japan, Language: English
I offer: Cadystin, Phytochelatin, Cd-binding peptides, Schizosaccharomyces pombe, Pichia pastoris, Bile salt stimulated lipase (BSSL, BAL), Midkine, Pleiotrophin, Fermentor culture, Nagoya University, Aichi, Biochemistry, Molecular Biology, Molecular Genetics, Human Genetics, Bioengineering
Akira Murasugi @ Odawara

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Akira Murasugi @ Odawara
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45 results for Akira Murasugi

Full text PDFs of our articles on the web.

Our PDF full articles on the web. Murasugi, A., and Hayashi, H. (1975) Purification and properties of leucyl-tRNA synthetase from Candida utilis. Eur. J. Biochem. 57, 169-175. http://onlinelibrary.wiley.com/doi/10.1111/ j.1432-1033.1975.tb02287.x/pdf Murasugi, A. and Takemura, S. (1978) Nucleotide sequence of leucine transfer RNA 1 from Candida (Torulopsis) utilis. J. Biochem. 83, 1029-1038. https://www.jstage.jst.go.jp/article/bioche mistry1922/83/4/83_4_1029/_pdf Murasugi, A., Wada, C., and Hayashi, Y. (1981) Cadmium-binding peptide induced in fission yeast, Schizosaccharomyces pombe. J. Biochem. 90, 1561-1564. https://www.jstage.jst.go.jp/article/bioche mistry1922/90/5/90_5_1561/_pdf Murasugi, A., Wada, C., and Hayashi, Y. (1983) Occurrrence of acid-labile sulfide in cadmium-binding peptide 1 from fission yeast. J. Biochem. 93, 661-664. https://www.jstage.jst.go.jp/article/bioche mistry1922/93/2/93_2_661/_pdf Murasugi, A., Nakagawa, C.W., and Hayashi, Y. (1984) Formation of cadmium-binding peptide allomorphs in fission yeast. J. Biochem. 96, 1375-1379. https://www.jstage.jst.go.jp/article/bioche mistry1922/96/5/96_5_1375/_pdf Hayashi, Y., Nakagawa, C.W., and Murasugi, A. (1986) Unique properties of Cd-binding peptides induced in fission yeast, Schizosaccharomyces pombe. Envirom. Health Perspect. 65, 13-19. http://www.ncbi.nlm.nih.gov/pmc/articles/PM C1474681/pdf/envhper00436-0018.pdf Murasugi, A., Takemori, N., and Hashimoto, S. (1987) Discrimination and quantitative analysis of wild type and point mutant early region 1B gene of adenovirus using oligonucleotide hybridization probes. J. Biochem. 102, 627-633. https://www.jstage.jst.go.jp/article/bioche mistry1922/102/3/102_3_627/_pdf Murasugi, A. (1989) A model for the initiation of translation at the late stage after infection by adenoviruses. Biochem. J. 260, 621-622. http://www.ncbi.nlm.nih.gov/pmc/articles/PM C1138717/pdf/biochemj00206-0299.pdf Tanaka, S., Ko, K., Kino, K., Tsuchiya, N., Yamashita, A., Murasugi, A., Sakuma, S., and Tsunoo, H. (1989) Complete amino acid sequence of an immunomodulatory protein, Ling Zhi-8 (LZ-8). J. Biol. Chem. 264, 16372-16377. http://www.jbc.org/content/264/28/16372.lon g Murasugi, A., Tanaka, S., Komiyama, N., Iwata, N., Kino, K., Tsunoo, H., and Sakuma, S. (1991) Molecular cloning of a cDNA and a gene encoding an immunomodulatory protein, Ling Zhi-8 (LZ-8) from a fungus, Ganoderma lucidum. J. Biol. Chem. 266, 2486-2493. http://www.jbc.org/content/266/4/2486.long Asami, Y., Nagano, H., Ikematsu, S., and Murasugi, A. (2000) An approach to the removal of yeast specific O-linked oligo-mannoses from human midkine expressed in Pichia pastoris using site-specific mutagenesis. J. Biochem. 128, 823-826. https://www.jstage.jst.go.jp/article/bioche mistry1922/128/5/128_5_823/_pdf Murasugi, A., and Aiba, Y. (2001) Comparison of three signals for secretory expression of recombinant human midkine in Pichia pastoris. Biosci. Biotechnol. Biochem. 65, 2291-2293. https://www.jstage.jst.go.jp/article/bbb/65 /10/65_10_2291/_pdf Murasugi, A., and Aiba, Y. (2002) Characterization of partially truncated human midkine expressed in Pichia pastoris. Biosci. Biotechnol. Biochem. 66, 1295-1300. https://www.jstage.jst.go.jp/article/bbb/66 /6/66_6_1295/_pdf Murasugi, A., Kido, I., Kumai, H., and Asami, Y. (2003) Efficient production of recombinant human pleiotrophin in yeast, Pichia pastoris. Biosci. Biotechnol. Biochem. 67, 2288-2290. https://www.jstage.jst.go.jp/article/bbb/67 /10/67_10_2288/_pdf Murasugi, A. Asami, Y., Kido, I., and Kumai, H. (2005) High level secretory expression system of intact MK family ptotein. United States Patent 6,867,037. http://www.freepatentsonline.com/6867037.pd f 村杉 章(2010年)Phytochelatin:生合成機構、光反応性半導体製造への応 用、及び生物学的環境指標としての利用.生物工学、第88巻、11号、616. http://www.sbj.or.jp/wp-content/uploads/fil e/sbj/8811_biomedia_5.pdf Murasugi, A. (2011) Heavy metal binding peptides, cadystin (phytochelatin), induced in Schizosaccharomyces pombe: Biosynthesis, fabrication of photoresponsive semiconductors, and detection of heavy metal environmental pollution ̶Review̶ Seibutsu-kogaku, 89, 228-237. https://www.sbj.or.jp/wp-content/uploads/fi le/sbj/8905/8905_review.pdf Murasugi, A. (2011) Secretory expression of human proteins in Pichia pastoris: Production of midkine, pleiotrophin, and bile salt-stimulated lipase in controlled fermentor. Seibutsu-kogaku, 89, 570–583. http://www.sbj.or.jp/wp-content/uploads/fil e/sbj/8910/8910_hobun.pdf
Akira Murasugi @ Odawara
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yasni 2012-10-16  +  

The summary of my article published in 2010 has been placed in a book.

My article “Secretory expression of human protein in the yeast Pichia pastoris by controlled fermentor culture. Recent Pat Biotechnol, 4, 153-166 (2010)” has been placed as the third topic in the electronic book of “Pancreatic Hormones: Advances in Research and Application: 2011 Edition, ScholarlyBrief” Edited by Q. Ashton Acton Published by ScholarlyEditions, Atlanta, Georgia
Akira Murasugi @ Odawara
10x
yasni 2012-06-27  +  

Evaluation of My Research; Application of Science Metrics

1  Personal productivity index calculated from the data of my original articles.   Number of article: 21 Number of first author article: 17 Number of forth author article: 1 Number of fifth author article: 2 Number of sixth author article: 1   *Uniform up-index = 7.875 *Golden ψp-index = 11.005 These are calculated according to Assimakis N and Adam M (A new author’s productivity index: p-index, Scientometrics (2010) 85: 415-427)   2  Mean citation number   *Mean citation number of the original articles shown in Google Scholar data = 32.8      
Akira Murasugi @ Odawara
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yasni 2012-02-10  +  

Review article in Japanese with English abstract has been open in the web-site at 25 Oct 2011

Review title; Secretory expression of human proteins in Pichia pastoris: Production of midkine, pleiotrophin, and bile salt-stumulated lipase in controlled fermentor.
Akira Murasugi @ Odawara
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sbj.or.jp 2011-10-25  +  

Immunomodulatory protein cloned from ... - Google Patents

Our paper is cited in this patent.
Akira Murasugi @ Odawara
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google.co.uk 2011-06-26  +  

My publications are here (3)

41  Murasugi, A. (2008) Cadystin (phytochelatin) synthesis induced with cadmium and resulted formation of cadmium sulphide nanoparticles in Schizosaccharomyces pombe. Current Topics Biotech. 4, 65-73. 42 Murasugi, A. (2008) Optimized production of recombinant human proteins in fermentor cultures of the yeast, Pichia pastoris. Current Topics Biotech. 4, 101-108. 43 Murasugi, A. (2008) [Metabolic syndrome and obesity.], in [“Obesity in Japan”], Functional Food Research Committee, ILSI Japan 44 Murasugi, A. (2009) [Improvement of oral health with milk], Food Style 21, 13, 40-41. 45 Murasugi, A. (2010) Secretory expression of human protein in the yeast Pichia pastoris by controlled fermentor culture. Recent Pat Biotechnol, 4, 153-166. 46 Murasugi, A. (2010) [phytochelatin: Biosynthesis, fabrication of photoresponsive semiconductors, and use for biological indicator to detect environmental pollution.], Seibutsu kogaku, 88, 616. 47 Murasugi, A. (2011) [Heavy metal binding peptides, cadystin (phytochelatin), induced in Schizosaccharomyces pombe: Biosynthesis, fabrication of photoresponsive semiconductors, and detection of heavy metal environmental pollution.], Seibutsu kogaku, 89, 228-237. 48 Murasugi, A. (2011) [Secretory expression of human proteins in Pichia pastoris: Production of midkine, pleiotrophin, and bile salt stimulated lipase in controlled fermentor.], Seibutsu kogaku, 89, 570-583. 49   Murasugi, A. (2013) Efficient expression and purification of recombinant therapeutic protein candidates, human midkine and pleiotrophin. Current Pharm Biotechnol, 14, 768-784.
Akira Murasugi @ Odawara
3x
yasni 2011-05-31  +  

My publications are here (2)

21    Wallace, R.B., Studencki, A.B., and Murasugi, A. (1987) Biotinylated oligonucleotide hybridization probes: progress towards a non-radioactive method for the diagnosis of human genetic diseases. GBF Monogr. Ser. 8 (Chem. Synth. Mol. Biol.) 95-105. 22    Murasugi, A., and Hashimoto, S. (1988) Cloning of adenovirus 2 terminal DNAs using the primer extension method. Nucl. Acid Res. Symp. Ser. 20, 55-56. 23    Murasugi, A. (1988) [A competitor improves the discrimination ability of a oligonucleotide probe for a point mutation.] Seikagaku, 60, 48-50. 24    Ikeda, M., Sakakibara, H., Taga, M., Uemura, T., Minaguchi, H., Murasugi, A., and Hshimoto, S. (1988) [Gene Expression of Gonadotropin Releasing Hormone in the Rat Hypothalamus.], Nihon Sanfujinka Gakkaishi, 40 (Supplement), S-312. 25     Murasugi, A. (1989) A model for the initiation of translation at the late stage after infection by adenoviruses. Biochem. J. 260, 621-622. 26 Tanaka, S., Ko, K., Kino, K., Tsuchiya, N., Yamashita, A.,  Murasugi, A., Sakuma, S., and Tsunoo, H. (1989) Complete amino acid sequence of an immunomodulatory protein, Ling Zhi-8 (LZ-8). J. Biol. Chem. 264, 16372-16377.   27    Murasugi, A., Tanaka, S., Komiyama, N., Iwata, N., Kino, K., Tsunoo, H., and Sakuma, S. (1991) Molecular cloning of a cDNA and a gene encoding an immunomodulatory protein, Ling Zhi-8 (LZ-8) from a fungus, Ganoderma lucidum. J. Biol. Chem. 266, 2486-2493. 28    Murasugi, A., Aiba, Y., and Asami, Y. (2000) Production of recombinant human midkine in yeast, Pichia pastoris. J. Biosci. Bioeng. 90, 395-399. 29    Asami, Y., Nagano, H., Ikematsu, S., and Murasugi, A. (2000) An approach to the removal of yeast specific O-linked oligo-mannoses from human midkine expressed in Pichia pastoris using site-specific mutagenesis. J. Biochem. 128, 823-826. 30    Murasugi, A., and Aiba, Y. (2001) Comparison of three signals for secretory expression of recombinant human midkine in Pichia pastoris. Biosci. Biotechnol. Biochem. 65, 2291-2293. 31    Murasugi, A., Asami, Y., and Kikuchi, Y. (2001) Production of recombinant human bile salt-stimulated lipase in Pichia pastoris. Protein Expr. Purif. 23, 282-288. 32    Murasugi, A. (2001) [Alpha-linolenic acid], in [“Functional Food Science in Japan”], pp.69-71, Functional Food Research Committee, ILSI Japan 33    Murasugi, A. (2001) Alpha-linolenic acid, in “Functional Food Science in Japan”, pp.45-46, Functional Food Research Committee, ILSI Japan 34    Murasugi, A., and Aiba, Y. (2002) Characterization of partially truncated human midkine expressed in Pichia pastoris. Biosci. Biotechnol. Biochem. 66, 1295-1300. 35    Murasugi, A. (2002) [Heterologous protein expression in microorganisms.], [Experimental Procedures after genome sequence era.] (Ooshima, T., Suzuki, K., Fujii, Y., and Muramatsu, T.), Volume II: [Procedure for sample preparation.], pp.55-62, Tokyo kagaku dojin 36    Murasugi, A., and Aiba, Y. (2003) Production of native recombinant human midkine in the yeast, Pichia pastoris. Protein Expr. Purif. 27, 244-252. 37  Murasugi, A., Kido, I., Kumai, H., and Asami, Y. (2003) Efficient production of recombinant human pleiotrophin in yeast, Pichia pastoris. Biosci. Biotechnol. Biochem. 67, 2288-2290. 38   Murasugi, A. (2004) [Diabetes and anti-oxidant ingredient.], in [“Functional Food and Health Claims”], pp.75-81,  Functional  Food Research Committee, ILSI Japan 39  Murasugi, A. Asami, Y., Kido, I., and Kumai, H. (2005) High level secretory expression system of intact MK family ptotein. United States Patent 6,867,037. 40  Murasugi, A. (2008) Optimized productions of recombinant human proteins, an enzyme and two cytokines, in fermentor cultures of the yeast, Pichia pastoris. An Invited Presentation in Ehrlich II – 2nd World Conference on Magic Bullets, Nürnberg, Germany, A-223 in Abstract Book.  
Akira Murasugi @ Odawara
3x
yasni 2011-05-30  +  

Canada patent application

HIGH LEVEL SECRETORY EXPRESSION SYSTEM OF INTACT MK FAMILY PROTEIN
Akira Murasugi @ Odawara
3x
brevets-patents.ic.gc.ca 2011-05-27  +  

Google Scholar search result for Akira Murasugi

Number of citations for each paper can be known.
Akira Murasugi @ Odawara
2x
scholar.google.co.uk 2011-05-21  +  

An Invited Presentation in Ehrlich II

Optimized productions of recombinant human proteins, an enzyme and two cytokines, in fermentor cultures of the yeast, Pichia pastoris. An Invited Presentation in Ehrlich II – 2nd World Conference on Magic Bullets, Nürnberg, Germany, Page A-223 in Abstract Book.
Akira Murasugi @ Odawara
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ehrlich-2008.org 2011-05-09  +  

Schizosaccharomyces pombe

Dateiformat: PDF/Adobe Acrobat - Schnellansichtvon Y Hayashi - 1986 - Zitiert durch: 20 - Ähnliche ArtikelAkira Murasugi*. Metallothioneins, a class of low molecular weight cysteine-rich proteins that bind heavy metal ions, have been found in various eucaryotic ...
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ncbi.nlm.nih.gov 2011-01-11  +  

Research Trends | Abstract

Optimized productions of recombinant human proteins in fermentor ... Akira Murasugi. Pages: 101 - 108. Number of pages: 8. Current Topics in Biotechnology. Volume ...
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researchtrends.net 2011-01-08  +  

Bentham Science Publishers

pp.153-166 (14) Author: Akira Murasugi. Abstract: The heterologous protein expression system of Pichia pastoris is now widely used for expression ...
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benthamdirect.org 2011-01-08  +  

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